产品详情
产品名称GFAP Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of total GFAP protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human GFAP .
基因/蛋白名称GFAP
别名GFAP; FLJ45472;
数据库入口号Swiss-Prot:P14136
NCBI Gene ID:2670
UniprotP14136
实际分子量50KD
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:100
Western blot analysis of extracts of mouse brain and mouse spinal cord, using GFAP antibody.
Immunohistochemical analysis of paraffin-embedded H-brain using GFAP antibody.
Immunohistochemical analysis of frozen neuroglial cells using GFAP antibody.
Immunohistochemical analysis of paraffin-embedded human kidney using GFAP antibody at dilution of 1:200 (200x lens). /Immunofluorescent analysis of hippocampal region of mouse using GFAP antibody.
Immunohistochemical analysis of paraffin-embedded human lung cancer using GFAP antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of hippocampal region of mouse using GFAP antibody.
背景
The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in non-myelin-forming Schwann cells in the peripheral nervous system (3).
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